Abstract: Paleopressure can be obtained by oil inclusion thermodynamics modeling, and ratio of vapor to liquid phase volume of oil inclusion is a sensitive parameter in this modeling, which can be got by 2D plane analysis and 3D shape reconfiguration of petroleum inclusions.The case study shows that within a certain oil conclusions shape, this ratio obtained by 2D plane analysis and 3D shape reconfiguration has relative error within ±10%, compared with the ratio given by confocal laser scanning?fluorescence scanning microscopy (CLFSM). WithoutCLFSM, the 2D plane analysis and 3D shape reconfiguration method is better than ocular estimation measurement method in calculation of the ratio, and can meet the accurate requirement for trapping pressure modeling of petroleum inclusions