Abstract: Mosuowan swell in the hinterland of Junggar basin which was located in the high position of the structural anticline during Cretaceous is the favorable area for hydrocarbon migration and accumulation. But little evidence can prove the hydrocarbon accumulation in the paleo-reservoir in Mosuowan swell. This paper selectes the typical exploration Well Moshen-1 in the Wellblock Pencan-2 in Mosuowan swell of Junggar basin, correlates and analyzes 25 cutting samples from the Sangonghe formation of Lower Jurassic in detail using quantitative grain fluorescence technique, and restores the complex reservoir charging process in Wellblock Pencan-2 based on the combined study of log data, structural development, hydrocarbon generation and expulsion history and hydrocarbon accumulation periods. The results show that paleo-reservoir occured in the second member of Sangonghe formation during Cretaceous with the water-oil contact (WOC) at 4 456 m in depth. Himalayan movement resulted in the leakage of the paleo-reservoir at the end of Paleogene and the WOC rose to 4 412 m. In Neogene, Jurassic source rocks charged with oil and gas again resulted in the present WOC at 4 440 m in depth (lower than WOC of the Paleogene) and allowing oil and gas accumulation to appear in sand bodies of the first member of Sangonghe formation under the conditions of no paleo-reservoirs. The research result provides new evidence for the study on hydrocarbon accumulation history of Sangonghe formation in Mosuowan swell