Abstract: A set of tuffite hydrocarbon source rocks developed in the second member of Permian Lucaogou formation, Santanghu basin, is characterized by large thickness, high abundance of organic matters, good organic matter type, and being in the oil generation window. Self-generation and self-storage is the main hydrocarbon accumulation pattern, source rocks are the good pay zones which have the typical characteristics of hydrocarbon accumulation in source rocks. Evaluation of movable hydrocarbon content in reservoirs should be focused on during source-rock oil exploration. The paper discusses the qualitative and quantitative characterization of movable hydrocarbons in the source-rock oil by using mud log, quantitative fluorescence, rock pyrolysis and NMR technologies. The movable hydrocarbon resources are estimated quantitatively with rock pyrolysis method. The result shows that the movable hydrocarbon resource in the source-rock oil of Permian Lucaogou formation in Malang sag, Santanghu basin is 5.53×108 t, resource abundance reaches 300×104 t/km2, which demonstrates that there is great potential for source-rock oil exploration.