The microbial dolomite of the Sinian Dengying formation in the Penglai gas field, central Sichuan Basin, is an important option for achieving additional reserves and production of oil and gas in deep to ultra-deep marine carbonate rocks in the basin. However, it is challenging to identify the impacts of reservoir diagenesis and pore evolution on hydrocarbon charging in this area due to its complex history of reservoir evolution. To objectively understand the potential and direction of petroleum exploration in the Penglai gas field, based on previous research results and exploration practice, together with comprehensive analysis of core thin sections, cathodoluminescence (CL) and trace elements, the relationship between diagenesis and hydrocarbon charging in the Dengying formation in the Penglai gas field was determined. The results show that the reservoirs of the Dengying formation in the Penglai gas field have been reworked by multiple stages of various diagenetic processes, including dissolution, cementation and filling, compaction-pressure-dissolution, recrystallization, tectonic disruption, and silicification. The diagenetic evolution process of the Dengying formation in the Penglai gas field includes five stages: contemporaneous-penecontemporaneous cementation, shallow-burial early diagenesis, uplifted-exposed epidiagenesis, burial diagenesis, and deep-burial late diagenesis. Ancient oil reservoirs were developed in the second member of Dengying formation (Deng 2 member) during the Caledonian period. The diagenetic minerals records three phases of hydrocarbon charging: the first phase is the cementation of fibrous dolomite (FD) cements around grape lace-like lattice pores, which emits no light or dim light; the second phase is the replacement of algal ring and fibrous dolomites by atmospheric freshwater dolomite (AFD) cements, which emits dim light; and the third phase is the filling of silty-fine granular dolomite (GD) cements into intergranular pores, intragranular pores, and lattice pores, which emits dim to dull-red light. The reservoir of the fourth member of Dengying formation (Deng 4 member) is filled with dolomite cements of multiple stages, with the reservoir subjected to multiple phases of hydrocarbon charging, including two phases of asphalt charging: the first phase, the Caledonian, exhibits low abundance, while the second phase, the Yanshanian, shows high abundance.