Abstract: With the increasing of demand for high-density acquisition, the vibroseis prospecting technologies of high-efficiency, high-fidelity and environmental protection have come into use, of which, the sliding scan acquisition can shorten the slip time between two neighboring shots and improve the efficiency of acquisition, but its harmonic distortion can reduce the quality of seismic data for the impact of the latter shot on the former one. This paper presents the mechanism of vibroseis harmonic noise and proposed a method for frequency-division harmonic noise suppression based on energy difference. The seismic data processing and superposition imaging results show that this method can obviously suppress the harmonic noise, protect effective signals and improve the signal/noise ratio