Abstract: In order to further illuminate the oil and gas distribution of the Devonian Donghe sandstone, and to explicitly determine the oil-gas exploration direction in Tarim basin, the paper analyzes the data related to the hydrocarbon accumulation in Donghe sandstone, and studies its hydrocarbon accumulation characteristics and oil-gas distribution. It is concluded that the oil and gas distribution in Donghe sandstone of Tarim basin is controlled by fault belt of Hercynian movement period and stratigraphic pinchout. Wether the traps constrained by the structures of Hercynian movement period could trap oil and gas or not depends on the trap sizes and the distance from oil source, and the eastern section of the Selibuya fault belt is the favorable area for oil and gas exploration. Wether the traps controlled by stratigraphic pinchout could trap oil and gas or not depends on the lithologies of the trap floor and their positions in structures, and the southern and northern sides of Shuntuoguole low uplift and the northern Awati depression are the most favorable areas for oil and gas exploration