Abstract: The effect of field test by microbial enhanced oil recovery (MEOR) showed its large fluctuation, unknown causes for success or failure, and hard to determine the microbial's adaptability to reservoirs, stimulation performance and applicable potentials. For these real reasons, the microbial DNA gene detection technique is developed, which provides an effective tool for investigating target microbial's genus, concentration, adaptability to reservoir, recovery performance and field application potential. By field test of well stimulation, paraffin removal and microbial flooding, together with systematic analysis of trace detection of the target microbial genus, major elements of affecting and restricting every link to the test are proposed. These are as a guide for technological modification, optimal scheme design and predominating test proceeding along with in-depth research and extensive application of MEOR.

Key words: microbe oil recovery, bacterial genus, gene, detection, field test, application