Abstract: Sikeshu sag is located in the intersection area of the northwestern and southern margins of Junggar basin. Because of the complex structural deformation, ascertaining traps in the area is vital for petroleum exploration. Based on 2D and 3D seismic geometry and kinematics analysis and combined with regional structural mapping, the research adopts the ideas and techniques of structural modeling to build a deformation model of "two periods of superimposition and double layered structure" for Sikeshu sag. It is considered that deep and shallow structural layers are developed in the sag by taking the Cretaceous Tugulu mudstone and the detachment layer of mudstone of Paleogene Anjihaihe formation as boundaries. The deep structural layer is mainly composed of Mesozoic NW-SE trending strike-slip faults and en echelon-like folds and the shallow structural layer is a Cenozoic detachmetn thrust structure. The two periods of structures superimposes vertically and exhibit a Mesozoic en echelon-like structural framework laterally. Based on the comprehensive research of oil and gas accumulation, a hydrocarbon accumulation model of vertical superimposition is proposed for Sikeshu sag. The reservoir in the shallow structural layer is influenced by fault connection and its distribution is controlled by Cenozoic faults; the deep structural layer is very near to hydrocarbon generation center in which structural traps formed early and late deformation was weak, which benefits long-term hydrocarbon migration. The commercial oil and gas flow with the daily production more than 1 000 tons obtained from the Well Gaotan-1 in Jan., 2019 can prove that the hydrocarbon accumulation conditions of Sikeshu sag is favorable