Abstract: To understand the rules of flow in shale oil reservoir, taking the Lucaogou formation of Jimsar sag in Junggar basin as an example, the micro-pore structures and flow mechanism of the shale oil reservoir were studied by means of laboratory physical experiments and digital core technology. Based on the physical experiment of single-phase flow through shale oil cores, a non-linear mathematical model of single-phase flow based on starting pressure gradient and an empirical formula of permeability related to pore structure parameters were obtained. Digital core technology and Lattice Boltzmann method were used to simulate the single-phase fluid of shale oil and to realize the fine characterization of fluid flow. The results show that the relation between seepage velocity and pressure gradient in the shale oil reservoir has a typical non-darcy flow characteristic, which is a cubic function relation with a good correlation.