新疆石油地质

新疆石油地质 ›› 2003, Vol. 24 ›› Issue (2): 164-166+189.

• 应用技术 • 上一篇    下一篇

DNA 基因检测技术在微生物采油中的应用

吕振山1, 邸胜杰1, 王利峰1, 张卫帼2   

  1. 1.中国石油 吉林油田分公司 勘探开发研究院,吉林 松原 138003;
    2.新疆油田分公司 勘探开发研究院, 新疆 克拉玛依 834000
  • 收稿日期:2002-02-09 修回日期:2002-08-12 出版日期:2003-04-01 发布日期:2020-09-17
  • 作者简介:吕振山（1967-）,男,吉林梨树人,高级工程师,现从事微生物实验及采油研究。联系电话：0438-6335903

Application of DNA Gene Detection Technique in the Microbial Enhanced Oil Recovery

LV Zhen-shan, DI Sheng-jie, WANG Li-feng, ZHANG Wei-guo   

  • Received:2002-02-09 Revised:2002-08-12 Online:2003-04-01 Published:2020-09-17
  • About author:LV Zhen-shan (1967-), Male, Senior Engineer, MEOR Experimental Study,Research Institute of Exploration and Development, PetroChina Jilin Oilfield Company, Songyuan, Jilin 138003, China

PDF

243

摘要： 针对微生物采油矿场试验效果波动性大,成功与失败要因不清,菌种油藏适应能力、增产效果及应用潜力难以确定等实际问题,开发建立了菌种DNA基因检测技术。为考察研究目的菌的属种、含量、纯度、油藏适应性、采油性能、矿场应用潜力等实验研究,提供了有效的技术手段。通过油井吞吐、清蜡、微生物驱等矿场试验,目的菌种基因跟踪检测的系统分析,说明了影响和制约矿场试验各环节的主要因素,为调整技术工艺、优化方案设计、把握试验进程提供了依据;为微生物采油技术的深入研究和矿场大规模应用指明了方向。

关键词: 微生物采油, 菌种, 基因, 检测, 现场试验, 应用

Abstract: The effect of field test by microbial enhanced oil recovery (MEOR) showed its large fluctuation, unknown causes for success or failure, and hard to determine the microbial's adaptability to reservoirs, stimulation performance and applicable potentials. For these real reasons, the microbial DNA gene detection technique is developed, which provides an effective tool for investigating target microbial's genus, concentration, adaptability to reservoir, recovery performance and field application potential. By field test of well stimulation, paraffin removal and microbial flooding, together with systematic analysis of trace detection of the target microbial genus, major elements of affecting and restricting every link to the test are proposed. These are as a guide for technological modification, optimal scheme design and predominating test proceeding along with in-depth research and extensive application of MEOR.

Key words: microbe oil recovery, bacterial genus, gene, detection, field test, application

中图分类号: