In recent years, with the progress of oil and gas exploration in the Tarim basin, large-scale strike-slip fault systems have been discovered in the Paleozoic strata of the platform area in the basin and a new type of fault-karst reservoir has been identified. Due to multiple tectonic movements in the basin, these strike-slip faults exhibit multilayer structures featured with multiple phase superimposition. Based on high-quality 3D seismic data, drilling data, and petroleum geological data, the multilayer superimposition of large-scale strike-slip faults in the basin and its controls over hydrocarbon accumulation were investigated. The research results show that the strike-slip fault zones in the platform area of the Tarim basin primarily develop five structural layers in the Paleozoic: Lower Cambrian pre-salt structural layer, Middle Cambrian salt structural layer, Upper Cambrian-Middle Ordovician carbonate structural layer, Upper Ordovician-Carboniferous clastic structural layer, and Permian magmatite structural layer. Affected by multiple tectonic movements and strike-slip fault activities, these layers exhibit characteristics of banded spatial distribution, vertical superposition, and differential superimposition. The superimposition patterns can be broadly categorized into four types: connection, overlapping, inverse superimposition, and inverse reformation. These superimposition patterns have significant impacts on hydrocarbon accumulation, and three types of reservoirs such as TypeⅠ (Ordovician carbonate reservoirs), Type Ⅱ (Ordovician carbonate, Silurian clastic, and Permian magmatite reservoirs), and Type Ⅲ (Cambrian pre-salt dolomite reservoirs) are formed.